COMPARATIVE PHYTOCHEMICAL SCREENING OF PHENOLOIDS IN LYSIMACHIA SPECIES
A comparative qualitative and quantitative screening of phenoloids of Lysimachia vulgaris L., Lysimachia nummularia L. and Lysimachia punctata L. is reported for the first time. The total flavonoid, hydroxy-cinnamic acid, tannin and polyphenol content of these species were examined by spectroscopic methods (Ph. Eur. 5.). The accumulation of the various phenoloids in individual organs was investigated as well. The quantitative analytical measurements revealed that the total flavonoid content expressed in hyperoside of L. vulgaris was significantly lower than of the samples of L. nummularia, and L. punctata, but its hydroxycinnamic acid content was outstanding. The accumulation of polyphenols and tannins showed to be homogeneous among the samples. The in vitro radical scavenging activities of the plant extracts were tested by DPPH and ABTS methods. Our studies proved that L. nummularia extract had the most significant activity. Investigations on the correlation between the composition of flavonoids in the samples and their in vitro antioxidant effect showed that the dominance of myricetin glycosides in L. nummularia is closely related with the most significant antioxidant capacity of this sample. In addition to the quantitative accumulation of flavonoids, significant differences have been observed in the composition of their quality as well. The main flavonoids in L. nummularia and L. vulgaris were identified as myricetin- and myricetin- quercetin-, and kaempferol-glyosides respectively using HPLC-DAD-ESI-MS/MS method.
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