HPLC METHOD DEVELOPMENT FOR DETERMINATION OF PYRAZINAMIDE AND RELATED SUBSTANCE BY USING QUALITY BY DESIGN (QBD) APPROACH
A robust and simplified high-performance liquid chromatography (HPLC) method was developed for the estimation of Pyrazinamide and
its related substance. A systematic approach, one of the parts of QbD (quality by design) was used in suitable analytical method
development. The HPLC segregation method was carried out with C-18 Column (3.9x300 mm, I. D. 10 µm), a mobile phase of phosphate
buffer: acetonitrile (pH 3.0) 90:10 v/v, detected at 270 nm. Optimization to this method was done by response surface methodology by
applying a three-level Box Behnken design with three center points.