E-ISSN 2063-5346
 

Research Article 


INVESTIGATION OF THE APOLIPOPROTEIN E GENE POLYMORPHISM VARIATIONS AND CORRELATION WITH ANTI-CYCLIC CITRULLINATED PEPTIDES ANTIBODIES IN IRAQI PATIENTS WITH RHEUMATOID ARTHRITIS

Montadhar K. Sultan, Moaed E. Al-Gazally, Sabah J. Al-Rubaie.

Abstract
Objective: To the assessment of Apolipoprotein E genetic polymorphism variations (E2/E3, E3/E4, E3/E2, E2/E4, E2/E2, E4/E4) and correlation with anti-cyclic citrullinated peptides (ACCP) antibodies in Iraqi patients with rheumatoid arthritis.
Methods: A case-control study was conducted at the rheumatologic outpatient department at Baghdad teaching hospital and included forty-five patients with rheumatoid arthritis (seven male and 38 female) enrolled in the study. APOE and ACCP levels were estimated by ELISA. RT-PCR was used to investigate APOE SNP (rs7412 and rs429358).
Results: The results of this study reveal the statistical difference in the level of Apo E between the patients and the control group. The mean of the APOE in the patient's group compared to the control group (p-value <0.001). The results also show a significant difference between the level of ACCP in the patient group compare to the control group (p-value <0.001 ). In the RA and control groups, the results of (rs7412 and rs429358) (T, C) genotyping showed that (17vs.11) carry homozygous genotype (E2/E2), whereas (52 vs. 61) homozygous genotype (E3/E3), and (16vs14) respectively homozygous genotype (E4/E4). Since the frequency in these groups is (5vs4) of heterozygous genotype (E2/E4) in both the RA and control groups. Heterozygous anthers were not present in the sample group (E2/E3, E3/E4). Since our sample had small subgroups of APOE E2/E4 (p-value>0.05).
Conclusion: There were significant differences in APOE and ACCP levels but not significant in genotyping analysis between RA and control groups.

Key words: Rheumatoid arthritis, APOE, ACCP, SNP


 
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Pubmed Style

Sultan MK, Al-Gazally ME, Al-Rubaie SJ. INVESTIGATION OF THE APOLIPOPROTEIN E GENE POLYMORPHISM VARIATIONS AND CORRELATION WITH ANTI-CYCLIC CITRULLINATED PEPTIDES ANTIBODIES IN IRAQI PATIENTS WITH RHEUMATOID ARTHRITIS. ECB. 2022; 11(11): 83-89. doi:10.31838/ecb/2022.11.11.009


Web Style

Sultan MK, Al-Gazally ME, Al-Rubaie SJ. INVESTIGATION OF THE APOLIPOPROTEIN E GENE POLYMORPHISM VARIATIONS AND CORRELATION WITH ANTI-CYCLIC CITRULLINATED PEPTIDES ANTIBODIES IN IRAQI PATIENTS WITH RHEUMATOID ARTHRITIS. https://www.eurchembull.com/?mno=131732 [Access: January 21, 2023]. doi:10.31838/ecb/2022.11.11.009


AMA (American Medical Association) Style

Sultan MK, Al-Gazally ME, Al-Rubaie SJ. INVESTIGATION OF THE APOLIPOPROTEIN E GENE POLYMORPHISM VARIATIONS AND CORRELATION WITH ANTI-CYCLIC CITRULLINATED PEPTIDES ANTIBODIES IN IRAQI PATIENTS WITH RHEUMATOID ARTHRITIS. ECB. 2022; 11(11): 83-89. doi:10.31838/ecb/2022.11.11.009



Vancouver/ICMJE Style

Sultan MK, Al-Gazally ME, Al-Rubaie SJ. INVESTIGATION OF THE APOLIPOPROTEIN E GENE POLYMORPHISM VARIATIONS AND CORRELATION WITH ANTI-CYCLIC CITRULLINATED PEPTIDES ANTIBODIES IN IRAQI PATIENTS WITH RHEUMATOID ARTHRITIS. ECB. (2022), [cited January 21, 2023]; 11(11): 83-89. doi:10.31838/ecb/2022.11.11.009



Harvard Style

Sultan, M. K., Al-Gazally, . M. E. & Al-Rubaie, . S. J. (2022) INVESTIGATION OF THE APOLIPOPROTEIN E GENE POLYMORPHISM VARIATIONS AND CORRELATION WITH ANTI-CYCLIC CITRULLINATED PEPTIDES ANTIBODIES IN IRAQI PATIENTS WITH RHEUMATOID ARTHRITIS. ECB, 11 (11), 83-89. doi:10.31838/ecb/2022.11.11.009



Turabian Style

Sultan, Montadhar K., Moaed E. Al-Gazally, and Sabah J. Al-Rubaie. 2022. INVESTIGATION OF THE APOLIPOPROTEIN E GENE POLYMORPHISM VARIATIONS AND CORRELATION WITH ANTI-CYCLIC CITRULLINATED PEPTIDES ANTIBODIES IN IRAQI PATIENTS WITH RHEUMATOID ARTHRITIS. European Chemical Bulletin, 11 (11), 83-89. doi:10.31838/ecb/2022.11.11.009



Chicago Style

Sultan, Montadhar K., Moaed E. Al-Gazally, and Sabah J. Al-Rubaie. "INVESTIGATION OF THE APOLIPOPROTEIN E GENE POLYMORPHISM VARIATIONS AND CORRELATION WITH ANTI-CYCLIC CITRULLINATED PEPTIDES ANTIBODIES IN IRAQI PATIENTS WITH RHEUMATOID ARTHRITIS." European Chemical Bulletin 11 (2022), 83-89. doi:10.31838/ecb/2022.11.11.009



MLA (The Modern Language Association) Style

Sultan, Montadhar K., Moaed E. Al-Gazally, and Sabah J. Al-Rubaie. "INVESTIGATION OF THE APOLIPOPROTEIN E GENE POLYMORPHISM VARIATIONS AND CORRELATION WITH ANTI-CYCLIC CITRULLINATED PEPTIDES ANTIBODIES IN IRAQI PATIENTS WITH RHEUMATOID ARTHRITIS." European Chemical Bulletin 11.11 (2022), 83-89. Print. doi:10.31838/ecb/2022.11.11.009



APA (American Psychological Association) Style

Sultan, M. K., Al-Gazally, . M. E. & Al-Rubaie, . S. J. (2022) INVESTIGATION OF THE APOLIPOPROTEIN E GENE POLYMORPHISM VARIATIONS AND CORRELATION WITH ANTI-CYCLIC CITRULLINATED PEPTIDES ANTIBODIES IN IRAQI PATIENTS WITH RHEUMATOID ARTHRITIS. European Chemical Bulletin, 11 (11), 83-89. doi:10.31838/ecb/2022.11.11.009