E-ISSN 2063-5346
 

Research Article 
ECB. 2021; 10(3): 155-166


METHODS of INTEGRAL MEMBRANE PROTEIN EXTRACTION and THEIR LIMITATIONS

Kallakuri A., Rajapakse H.E.

Abstract
Membrane proteins makeup around one-third of all proteins in the body and play important roles in signal transduction, selective molecular transmission and membrane integrity. They also serve as important drug targets. Therefore, accurate structural and functional analysis of membrane proteins is critical. The extraction and study of membrane proteins is limited due to their amphipathic nature, making them difficult to solubilize in aqueous media. Integral membrane proteins can be extracted from bio-membranes with detergents and purified into detergent micelles, bicelles, bilayers, or other forms of amphipathic molecules. These platforms do not necessarily mimic bio-membranes and, therefore, may not reflect the integral membrane proteins' accurate biological structure and function. Recent studies have introduced better representations of the bio-membrane, such as membrane mimicking nanoparticles, lipid nanodiscs (LNDs) and their variations, and artificial polymers. Due to some limitations described below, there are continuous efforts to develop better platforms that correctly resemble plasma membranes, leading to the correct biological structure and function of membrane proteins for in vitro studies. This mini-review comprehensively summarizes the recent developments of membrane proteins extraction, their advantages and disadvantages.

Key words: Hydrophobic; Lipids; Membrane Protein; Structural Analysis; Transmembrane


 
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Pubmed Style

A. K, H.E R. METHODS of INTEGRAL MEMBRANE PROTEIN EXTRACTION and THEIR LIMITATIONS. ECB. 2021; 10(3): 155-166.


Web Style

A. K, H.E R. METHODS of INTEGRAL MEMBRANE PROTEIN EXTRACTION and THEIR LIMITATIONS. https://www.eurchembull.com/?mno=52073 [Access: September 25, 2022].


AMA (American Medical Association) Style

A. K, H.E R. METHODS of INTEGRAL MEMBRANE PROTEIN EXTRACTION and THEIR LIMITATIONS. ECB. 2021; 10(3): 155-166.



Vancouver/ICMJE Style

A. K, H.E R. METHODS of INTEGRAL MEMBRANE PROTEIN EXTRACTION and THEIR LIMITATIONS. ECB. (2021), [cited September 25, 2022]; 10(3): 155-166.



Harvard Style

A., K. & H.E, . R. (2021) METHODS of INTEGRAL MEMBRANE PROTEIN EXTRACTION and THEIR LIMITATIONS. ECB, 10 (3), 155-166.



Turabian Style

A., Kallakuri, and Rajapakse H.E. 2021. METHODS of INTEGRAL MEMBRANE PROTEIN EXTRACTION and THEIR LIMITATIONS. European Chemical Bulletin, 10 (3), 155-166.



Chicago Style

A., Kallakuri, and Rajapakse H.E. "METHODS of INTEGRAL MEMBRANE PROTEIN EXTRACTION and THEIR LIMITATIONS." European Chemical Bulletin 10 (2021), 155-166.



MLA (The Modern Language Association) Style

A., Kallakuri, and Rajapakse H.E. "METHODS of INTEGRAL MEMBRANE PROTEIN EXTRACTION and THEIR LIMITATIONS." European Chemical Bulletin 10.3 (2021), 155-166. Print.



APA (American Psychological Association) Style

A., K. & H.E, . R. (2021) METHODS of INTEGRAL MEMBRANE PROTEIN EXTRACTION and THEIR LIMITATIONS. European Chemical Bulletin, 10 (3), 155-166.