Intraperitoneal instillation of local anesthetics minimizes postoperative pain after laparoscopic surgeries. AIM: To evaluate the use of intraperitoneal ropivacaine and bupivacaine in Laparoscopic Cholecystectomy patients as effective and safe modality for post-operative analgesia. MATERIALS AND METHODS: After taking written informed consent,90 patients undergoing laparoscopic cholecystectomy were randomized among three groups of 30 patients each. In Group A, patients were given 0.9% of normal saline as placebo, in group B, patients were instilled with 20 ml of ropivacaine (0.75%) and in group C, patients were instilled with 20 ml of bupivacaine (0.50%) in gall bladder fossa after the removal of gall bladder. Patients were then assessed for post-operative pain after Laparoscopic Cholecystectomy. RESULTS: Demographic profile was comparable in the three groups. The mean intensity of post-operative pain assessed by visual analog scoring scale was statistically significant in normal saline group as compared to ropivacaine group at 0 hours (p=0.000), at 4 hours (p=0.001) at 8 hours (p=0.000) and at 12 hours (p=0.029) and while comparing with bupivacaine it was statistically significant at 0 hours (p=0.000), at 4 hours (p=0.017) and at 8 hours (p=0.010). While as pain intensity in bupivacaine group was more but statistically non-significant than ropivacaine group at all time intervals. CONCLUSION: Both bupivacaine 0.50% and ropivacaine 0.75% instilled in the gall bladder fossa are effective for post-operative analgesia upto 12 hours.