Introduction: Urinary tract infections (UTIs) are common and can cause severe symptoms, such as frequent urination, burning feelings, and pelvic discomfort. The abuse of medicines for UTIs adds to the increasing problem of antibiotic resistance. The purpose of this study was to investigate the severity of UTI symptoms and frequency of antibiotic resistance in a tertiary care center in Pakistan. Methodology: A cross-sectional study was conducted in 2023 over 12 months period at a tertiary healthcare hospital in Pakistan. A sample size of 700 adult patients who presented with UTI symptoms was chosen using power analysis. Demographic information, clinical symptoms, medical history, and history of antibiotic use were obtained from medical records. Urine samples were analyzed for uropathogens that cause UTIs, and antibiotic susceptibility testing was used to determine resistance rates. Results: Of the 700 participants, 322 were female. The common symptoms include fatigue, pain, frequent urination, anxiety, and pelvic pain. The UTI severity was categorized as mild, moderate, or severe. In the "Mild" category, 377, 240, and 64 patients reported mild, moderate, and 64 reported severe fatigue, respectively. Most patients reported pain, high frequency of urination, and anxiety. Antibiotic resistance rates were as follows: Bactrim (trimethoprim/sulfamethoxazole), 1 in 3 uncomplicated UTIs, Ciprofloxacin, 1 in 5 uncomplicated UTIs, Other Common Antibiotics (combined), 1 in 5 uncomplicated UTIs. Conclusion: Antibiotic resistance in UTIs is a growing concern, with multidrug resistance becoming more common. Urgent action is required to overcome antibiotic resistance and provide effective treatment for patients with UTIs.