Background and Purpose: Students as an educated group can be effective in preventing new cases of thalassemia by providing public education and increasing public awareness. The purpose of this study was to investigate the knowledge and attitude of medical and non-medical students about premarital counseling and factors related to thalassemia. Research Method: In this descriptive-analytical cross-sectional study, the data about 700 medical and non-medical students of Iranshahr city in Iran in 2019 was collected through Hajian's standard questionnaire. The collected data were analyzed using one-sample t-test, Pearson's correlation test and two-way analysis of variance test in SPSS 22 software. A significance level of 0.05 was considered. Findings: The findings showed that 69% of students were studying in non-medical universities and 31% were studying in medical sciences universities. The knowledge and attitude of medical and non-medical students of Iranshahr about premarital counseling and factors related to thalassemia