Pathogenic microbes are increasingly resistant to unnatural antibiotics made by humans, leading to the search for substitute drugs. In this study, Moringa (Moringa peregrina) was tested to see if it had antimicrobial effects on a few commonly occurring bacteria, specifically Staphylococcus aureus and Enterobacter hormaechei. During September 2022 to May 2023, this experiment was conducted at the Department of Science and Arts of the University of Nizwa in collaboration with the Laboratory of Microbiology at the University of Nizwa to determine whether ethanolic extract of Moringa peregrina leaves, roots, and seeds could be effective against Enterobacter hormaechei and Staphylococcus aureus bacteria. A well- diffusion method and minimum inhibitory concentration (MIC) were used to test the antibacterial activity of Ethanolic extract at different concentrations (100, 200, 400, 600, 800, and 1000 g / ml). A dose-dependent inhibitory effect was noted against the test microorganisms. Moringa peregrina leaves showed the greatest inhibition zone against S. aureus. Moringa leaves, roots and seeds were found to have antibacterial effects against gram positive bacteria (S. aureus) in this study. In comparison with roots and seeds, Moringa peregrina leaves have better potential against S. aureus compared with roots and seeds due to their composition of phytochemical compounds. There is a need for further research to isolate and identify the antimicrobial agent in M. peregrina seed oil. A deeper investigation should be conducted into the antibacterial agent dosages of these plant parts, which may be used by the pharmaceutical industry. In continuing studies, I suggested using both ethanol and methanol as solvents and utilizing a variety of bacterial species to get further contrasts.