Earthworms benefit soil quality by destroying buildups invigorating microbial decay, further developing soil fruitfulness, and evolving the actual soil properties like soil conglomeration and invasion. The objective of this study is to examine the essential amino acid profiles of three earthworm species (Lampito marutii, Octochaetona serrata, Eudrilus eugeniae) and also to study the changes in soil chemical, physical, and macromineral compositions. In light of the nutritional analysis, it is possible to argue that these three species of earthworm can provide sufficient protein and other trace elements to satisfy daily requirements. Currently, the use of worms as a feed source for cultured aquatic animals is still experimental. The amino acid profiles of the night crawlers species were kept in this concentrate through High-performance Liquid Chromatography (HPLC) and their qualities were communicated in Rate (%), Mean standard deviation, and Coefficient of Variation (CV) % among three unique species. The percentage of essential and nonessential amino acids was studied for all three species individually using a histogram. The level of an aggregate sum of amino acids from the three species diminished arranged by O. serrata followed by E. eugeniae and then L. mauritii. While contrasting and environmental characters, endogeic species show the most noteworthy qualities followed by epigeic and anecic species individually. With the rising expenses of traditional protein sources, such as fishmeal and soybean feast, it might involve time before the monetary benefits of delivering worms as a modest wellspring of protein become obvious to numerous water culturists and feed makers.