Road traffic accidents (RTAs) constitute leading cause of death among adolescents as well as young adults making it an important public health problem. Road traffic injuries are now the leading killer among people aged 5-29 years. Annually, roughly 400,000 youngsters under 28 years age succumb to death in a road traffic crash accounting to about 1050 youngsters every day. Knowing the rules and regulations regarding road safety forms an important aspect in preventing RTAs. Objective: This study aimed at assessing the knowledge, attitudes, and practices regarding road safety among college students. Materials and Methods: A cross-sectional study was conducted among 1000 college students from Jaipur, using a pre-designed questionnaire to assess the awareness and practices regarding road safety. Results: The prevalence of the accidents was 20%. The majority of the participants (78%) were driving vehicles during accidents. 44% of the participants were admitted in the hospital following accident. The factors associated with accidents were broken roads (54%), night time (34%), raining (40%), slow traffic (46%), and fault in vehicle (42%), high speed (48%), poor street light (32%), using mobile phone (24%) and not wearing helmet (60%). Conclusions: Bringing about behaviour change regarding road safety measures through information, education, and communication activities and improving legislative measures of traffic rules will contribute in making people responsible citizens of the country which will eventually bring reduction in the sufferings and death due to RTAs.