Here in we reported the green and novel protocol for the synthesis of 2-iodo-imidazothiazole derivatives from substituted phenacyl bromides and substituted 2-amino-4-aryl thiazoles using BEC (pH 12.5, 10% by weight) as a recyclable heterogeneous catalyst in environmentally sustainable reaction medium PEG-400. The anti-microbial activity was evaluated by using using disc diffusion assay with Staphylococci Aureus (MTCC-96), Escherichia coli, (MTCC-1588), and Candida albicans (MTCC-3018). According to the antimicrobial results the compounds 3b, 3c, 3e, 3f, and 3k had good antimicrobial activity. This result helps us for the identification of lead compounds with a 50% inhibitory concentration (IC50) of 18 to 28 mM. Consequently, the molecular docking with PDB:2Q85 Crystal Structure of E. Coli and PDB:3u2d S. aureus GyrB ATPase shows all the molecules are well packed in the active sites of the selected protein. The derivatives were characterized using spectroscopic methods, Molecular docking and screened for antimicrobial strains. The current method has several advantages, including catalyst recyclability, superior product yield, a quicker reaction time, and the absence of hazardous reagents.