Antimicrobial resistance has become a recognized "worldwide menace." As indicated in a newly issued report from the World Health Organization, it was revealed that around 700,000 individuals succumb annually as a result of infections that do not respond to drugs. So the aim of this study was antibiotic resistance pattern amongst IPD patients in a tertiary care hospital. Methodology: In this study we included 1090 total cases. Among all 322 cases were positive growth rests were negative. Positive cases were further proceed to culture and sensitivity test. This study was conducted in Department of Microbiology at ESIC Medical College and Hospital, Joka, Kolkata. The duration of study was over a period of two year. Result: We noted that the highest number of cases were associated with urine, followed by pus, ET, blood, sputum, vaginal swab, wound, and CSF. Among the 322 cases of positive growth, 68 were attributed to gram-positive bacteria, with the remainder being gram-negative. Escherichia coli was the most prevalent pathogen, followed by MRSA, Klebsiella spp., ACB complex, Pseudomonas, Enterococcus, MRCONS, CONS, and other gram-negative bacteria. Conclusion: It can be concluded that utilization of antibiogram studies can assist in the appropriate selection of antibiotics for treatment, monitoring local patterns of antibiotic resistance, and pinpointing opportunities for enhancing antimicrobial stewardship and educational efforts