Biodiesel, a promising substitute as an elective fuel has acquired huge consideration because of the anticipated brevity of regular powers and natural concern. The usage of fluid fills, for example, biodiesel created from Jatropha oil by transesterification process addresses one of the most encouraging choices for the utilization of traditional non renewable energy sources. The Jatropha oil is changed over into jatropha oil methyl ester known as biodiesel arranged within the sight of homogeneous corrosive impetus. The actual properties, for example, thickness, streak point, Kinematic consistency, Cloud point and Spill point were found out for Jatropha oil and Jatropha methyl ester. Similar attributes study was additionally done for the diesel fuel for acquiring the pattern information for investigation. The qualities got from the Jatropha methyl ester is firmly coordinated with the upsides of regular diesel and can be utilized in the current diesel motor with practically no adjustment.