At the present time, the world is suffering from the increasing jeopardy of incurable diseases like cancer, tumour, diabetes, arthritis, heart, brain or immune dysfunction etc., specially caused by the free radical induced oxidative damages. Therefore, the modern research community is very highly fascinated about the research related to the substances having potential antioxidant activity as these compounds are capable of preventing the free radical chain reactions and thereby can avert the ailments caused by oxidative damages. For this, simple, cost friendly, convenient, reliable and experimentally viable antioxidant activity assessment methods are very much significant. Due to the existence of numerous free radicals or oxidant sources and different kinds of antioxidant species with highly diverse chemical and physical characteristics, mechanism of action of antioxidants differ from each other, depending upon the nature of the substances involved in the process or other experimental parameters. Therefore, quite a number of antioxidant activity evaluation methods have been reported, each with their own advantages and disadvantages and selection of proper method with respect to the antioxidant and free radical type considering other important reaction parameters is very much imperative in order to have accurate estimation of the antioxidant capacity of the samples under study. In this short review, the author has attempted to analyse the principles behind the mechanism of action of certain electron transfer based antioxidant activity determination assays along with their advantages and disadvantages.