In this study was investigate thatcystatin C and ADA as novel biomarkersfor chronic kidney disease. Cystatin C 1.3kDacompound which is filtered from the blood stream through the kidneys.Adenosine deaminase is one of purine metabolic enzyme which is involved in adenosine breakdown and in development and maintenance of immune system. chronic kidney disease patients were studied in two groups namely test group of chronic kidney disease patients and normal subjects of control group; each group consists of 30 subjects.Cystatin C and Adenosine deaminase levels were significantly increased in severe kidney disease patients when compared with normal subjects.We conclude that the concentration of Cystatin C and ADA activity is a useful index for the diagnosis of chronic kidney disease