Lack of access to medicines and inappropriate doses result in serious morbidity and mortality and result in significant patient harm in terms of poor patient outcomes and adverse drug reactions. Therefore WHO recommended rational use of medicines for all practitioners. Aim and objective: To study the effect of education in rational use of medicine among general practitioners Methodology: Present study is a cross‑sectional, questionnaire‑based study conducted among general practitioners. Total 40 general practitioners were studied. Pre test was conducted. A workshop was conducted regarding rational use of medicine. Post test was conducted. Response of participants were given score. Data was analysed with appropriate statistical tests. Results and discussion: The average score in pre test for all participants was 77.72 and 83.57 in post test. This difference was statistically significant (p<0.05). The average score in pre test for all participants was 77.72 and 83.57 in post test. This difference was statistically significant (p<0.05).