With a high incidence rate of 10.4% in India, diabetes is a significant health concern. A non-invasive diagnostic tool called dermatoglyphics can be used to find possible diabetes risk factors. The purpose of this study is to look into the dermatoglyphic pattern in South Indian diabetic people. Methods: Between January 2021 and December 2021, a cross-sectional study was carried out in a tertiary care hospital in South India. 100 patients with diabetes and 100 healthy controls were enrolled in the study. Both hands' dermatoglyphic patterns were documented using conventional methods. The SPSS program was used to examine the data. Results: The fingerprint patterns of diabetic participants and healthy controls significantly differed (p< 0.05). The frequency of arches was higher and the frequency of loops and whorls was lower in the diabetes participants. Additionally, diabetic subjects' mean total ridge count was significantly lower than that of healthy controls (p< 0.05). Conclusion: In comparison to healthy controls, the dermatoglyphic pattern of diabetes people in South India is markedly different. Diabetes may be indicated by the increased frequency of arches and the lower frequency of loops and whorls. The results need to be confirmed and the underlying mechanisms need to be investigated by other research.