In current studies, zinc oxide nanoparticle poly-(N-isopropylacrylamide)-conjugate (ZnO-NIPAM) has been synthesized with the assessment of its wound repair and bacterial inhibition properties.The developed conjugates were aimed to determine its antibacterial activity with enhanced stability.Combination of nanomaterials with organic molecules is done to improve their activity and stability..Topographic analysis of the nanoparticle was investigated usingScanning electron microscopy of field emission –FESEM, antibacterial activity was evaluated using standard well diffusion method and wound-heal assay was determined using in-vitro scratch assay. The results proved significant antibacterial properties along with potential wound healing characteristics.UV-Vis and FESEM analysis showed the particle characteristics in support to literature survey. The obtained results shall be applicable for different pharmaceutical and biomedical studies in future.