Background- The menarche is an important event in a woman’s lifecycle (Februanti, ,2020).Menstruation is closely associated with taboos and sociocultural restrictions. Unhygienic practices increase the risk of reproductive tract infections (RTI), which might lead to further complications if not treated at right time. (Beeva Baruah, 2022). Menstruation has deep impact on quality of education as it involves a learning constituent affected by environment and infrastructure of the school (Fehintola, 2017).With this background, the study was planned to assess adolescent school going girls’ knowledge of menstruation and menstrual hygiene as well as their practices of menstrual hygiene, Bhagini Nivedita Girls H.S School, Kailashahar, Unakoti Tripura. Aims- To Assess the awareness level and determined the methods of menstrual hygiene among school going adolescent girls. Materials and method- A cross-sectional study was conducted between April-May 2023 in Bhagini Nivedita Girls’ H.S School, Kailashahar, Unakoti Tripura among 100 of eligible adolescents aged between 10 and 19 years, using a random sampling technique. A predesigned, self-administered structured questionnaire was used to collect the information from the participants. Data analysis was done using excel sheet and the results were expressed in percentages. Result-5% of adolescent girls had inadequate knowledge level, 20% of them had moderate knowledge level and 75% of them have adequate level of knowledge. 63% of the adolescent girls were in the category of Good Practice, 37% were in the category of Poor Practice regarding menstrual hygiene. Conclusion- This study was undertaken to evaluate the knowledge and practice assess on menstruation hygiene among school going adolescent girls, Bhagini Nivedita Girl’s H.S School (Kailashahar, Unakoti Tripura). The current study has revealed that menstruation and menstrual hygiene knowledge is poor among adolescent girls and practices regarding menstrual hygiene are not optimal