Bacterial subculture is a essential method in medical microbiology that entails the increase and isolation of micro organism from affected person samples. This approach has developed drastically over the years, with enhancements in each the techniques of isolation and identity of bacteria. In this essay, we can speak the contemporary and beyond techniques for bacterial tradition in scientific microbiology. In the past, the number one technique for bacterial way of life concerned plating affected person samples onto solid increase media, together with blood agar or MacConkey agar, and incubating the plates at precise temperatures. This method allowed for the identity and isolation of micro organism primarily based totally on their colony morphology and biochemical properties. However, this approach had its limitations, because it changed into time-ingesting and required a giant quantity of understanding to interpret the outcomes accurately.