Hair loss, also called alopecia, is a disorder caused by an interruption in the body's cycle of hair production. Commonly it affects On the scalp. On average, the scalp has 100,000 hairs that cycle through periods of growing, resting, falling out, and regenerating. Grey hair is really hair with reduced melanin, while white hair completely lacks it. Coconut oil softens hair and increases shine. Black seed oil strengthens moisturizes the hair, adds nutrients, and stimulates the scalp. Rosemary oil performed a hair growth promoter, with less scalp itching as a side effect. Different oils can help to grow hair and also add strength and shine. In this present study the different herbs and oils was used to prepared herbal hair oil by using different formulation methods which contain major chemical compounds like ursolic acid diosgenin to improve hair growth and reduce grey hair. After the oil was evaluated by morphological and pharmacological studies. By using HPTLC and microbiological studies we found that two formulations gave good results compare with other formulations.