The spleen is a hemolymph organ in the human body. It is the largest and most clinically important lymphoid organ. The spleen can have a wide range of anomalies in its shape, location, number, and size. The accessory spleen is a common congenital anomaly that is due to congenital failure of the fusion of splenicules. The accessory spleen is mostly asymptomatic but can mimic certain tumors and also can lead to relapse post-splenectomy. Aim of the Study: The present study was undertaken to study the incidence, and microscopic structure of the accessory spleen in human adult cadavers and to compare the results with previous studies. Materials and Methods: The present study was done on 41 human adult cadaveric spleens obtained from the Department of Anatomy, J.N. Medical College, Belagavi. Accessory splenic tissues, if present, were noted, photographed, and studied histologically. Results: Accessory spleens were found in 7.32% of cases in the form of roundish nodules. They were observed at the hilum of the spleen. On microscopy of the accessory spleen, the white pulp was comparatively ill-defined, and the red pulp was predominant. Conclusion: These findings will be helpful for Surgeons, Radiologists, Oncologists, and to Anatomists too.