Terminalia bellirica is one of the most planted and utilized as traditional medicine around South-East Asia. The various conventional medical systems, including Ayurveda, Unani, Siddha, and traditional Chinese medicine, have each highlighted the benefits of fruits of this plant. The fruits of this plant contain glucoside, tannins, gallic acid, ethyl gallate, and chebulinic acid, which have anti-inflammatory, anti-cancer, antidiarrheal, anti-microbial, and antipyretic properties. Yet scientific evidence-based molecular mechanisms have not been fully reported. This present study has highlighted the antioxidative, antimicrobial, and anticancer properties of the ethyl acetate extract of Terminalia bellirica. Antibacterial and antifungal activity was carried out against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Saccharomyces cerevisiae, and Candida krusei respectively. Cytotoxicity studies were carried out for cell lines such as RAW 264.7, L6, and PA1. The results of this study demonstrate excellent efficacy against PA1 (ovarian cancer) cell lines.