Background: The pituitary gland is the master gland of the body. Due to its high soft tissue contrast, multi-planar capabilities, and lack of ionizing radiation, MRI is currently the preferred examination for pituitary evaluation. Aim: To study Age and gender related changes in dimensions & Volume of pituitary gland using MRI. Study design: prospective observational type. Material and Method: Assessment of 150 subjects in which 100 subjects were included with age ranging from 10-70 years in which 44 were males and 56 females. for measurement of gland based T1 MPRAGE sequence is used in all 3 aspects AP dimension, height, width of pituitary gland. The statistical analysis done by SPSS version 23.Independent t test, one way ANOVA and Pearson correlation of parameters were calculated. Result: Mean height of pituitary in female patients of each age group was greater as compare to males in all age group. the max height of gland reached in 31 to 40 in both genders. the maximum height of pituitary volume was noted between 10-20 years in females 406.68 ±65.41mm3 and least was noted in age 61 to 70 years 358.04±63.60 mm3.