Introduction: Human third molar improvement is extensively used to expect chronological age of sub person people with unknown or doubted age. Age associated overall performance of degrees and measurements had been as compared to evaluate if measurements introduced statistics to age predictions from third molar root formation stage Aim: The aim of the study is to determine the age estimation from third molar by the gleiser and hunt method Material and Method: The study sample consists of 100 OPG’s (50 male and 50 female) of age from 15 to 19 years with known date of birth. The data was collected from the department of forensic odontology, Saveetha dental college. Results: In the study it was observed that there is a difference between male and female roots . The standard deviation of for male in tooth 38 is ± 2.65 whereas in the female it is ± 3.44 . While comparing male and female, female is more accurate compared to male in tooth 38. Conclusion: It is concluded that the female has more root maturation compared to the male and also it is concluded that age can be estimated from the third molar by modified Gleiser and hunt method.