This study is design to examine the potential of agmatine on acute ethanol-induced behavioral alterations during adolescence. Acute ethanol exposure has been associated with range of disturbances, including, anxiety, depression, social impairment and cognitive deficits. Ethanol's impact on neurotransmitter systems, oxidative stress, and excitotoxicity can contribute to these alterations. Agmatine, a novel neurotransmitter and neuromodulator in brain, has shown promising results in preclinical studies for its multifaceted neuroprotective properties. It has been investigated for its potential to modulate neurotransmitter systems, and reduce oxidative stress. In this experiment, rats were subjected to acute ethanol administration from PND 34 - 41 and administered with agmatine (20, 40 and 80 mg/kg) to evaluate its effect on ethanol-induced behavioral alterations. The results from different behavioral paradigms showed that agmatine in doses 40 mg/kg and 80 mg/kg showed prominent anxiolytic and antidepressant effect. Additionally, it improved the recognition memory in object recognition task and also improved altered social behavior associated with acute ethanol exposure. Furthermore, biochemical analysis revealed involvement of neurotransmitter modulation antioxidant potential of agmatine by reducing enhanced glutamate levels, NO and lipid peroxidation and improving antioxidant defense and GABA levels in brain. The results of this study provide valuable insights into the potential benefits of agmatine in mitigating the acute behavioural consequences of ethanol exposure.