In the present study, we report the isolation from the soil of one of the slaughterhouses in the Tashkent region, the proteolytic bacterium Bacillus subtilis IMRUZ-7, which was chosen to optimize its cultivation conditions for protease production. This strain can be used for large-scale alkaline protease production to meet the needs of current industrial applications. Starch and corn broth are cheap sources of carbon and nitrogen, which led us to offer an inexpensive nutrient medium to maximize protease production. The optimum pH and temperature for enzyme activity were determined to be 9.0 and 60°C, respectively. Since the thermoactivity and pH stability of proteases are of great importance for industrial use, the enzymatic properties of Bacillus subtilis IMRUZ-7 protease indicate the potential use of this bacterium and its protease for various industrial purposes.