Nephrotoxicity is one of the primary side effects of cisplatin (CP), a powerful chemotherapeutic drug that is frequently used to treat cancer. Antioxidant and anti-inflammatory characteristics are found in the body hormone melatonin, which is secreted by the pineal gland. The purpose of the current study is to determine whether melatonin can protect rats from cisplatin-induced nephrotoxicity. Materials and Methods: Forty rats were used in this study and divided into four groups (10 rats for each): group I( control group) in which, the animals were injected intraperitonealy with normal saline), group II ( melatonin group)in which, the animals were injected intraperitonealy with melatonin (4mg/kg bw ip for 10 days)group III (cisplatin group) in which, the animals were injected intraperitonealy with (single dose of 7 mg/kg bw),and group IV (melatonin/cisplatin group) in which, the animals were injected intraperitonealy with melatonin for 10days+ cisplatin with a single dose on the 5th day of experiment at the above-said doses. At the end of experiment time, all rats were sacrificed and kidneys were dissected out for histological study.