An inter appointment flare-up caused by inflammatory reactions is one of the most important side effects of apical extrusion. The preparation endpoint, irrigation fluid type and quantity, instrumentation size, technique, and instrument type are only a few of the variables that determine the amount of extruded debris. Aim: The aim of this study was to evaluate the apical extrusion of debris during root canal instrumentation using two different rotary file system. Material and Method: Protaper gold rotary Ni-Ti system and Neo Endo rotary Ni-Ti system were used to instrument the two groups of 40 freshly extracted mandibular premolars with straight roots. Before instrumentation, preweighed Eppendorf tubes are adapted for each tooth. A 30-gauge needle and 1 mL of distilled water were used to irrigate the area after each instrument during instrumentation. After being taken out of the Eppendorf tubes, the tooth's tips were irrigated with 2 ml of distilled water. In each group, the total amount of irrigant was 8 ml. After 15 days of 68°C incubation, all tubes were weighed. Calculating the difference between the pre- and post-debris weights, statistical analysis was carried out using an independent t-test, with the threshold of significance set at 0.05. Result: The difference between pre- and post-weights was significantly greater for the neoendo system Conclusion: Although apical debris extrusion was induced by both rotary files, the protaper gold demonstrated noticeably less debris extrusion than the neoendo rotary file