The most common cause of vaginal infections is bacterial vaginosis, also known as non-specific vaginitis. In our country, it is difficult to detect the organism early on and begin the appropriate treatment. This is mainly due to a lack of awareness among reproductive aged females and missed or ignored follow-up during gynecological treatment. In this disease there is vaginal discharge, which can be itchy or not. It strongly correlates with IVF implantation rates, preterm labor, premature membrane rupture, and low birth weight during pregnancy. This study sought to determine the prevalence of bacterial vaginosis among women of reproductive age. A cross sectional study wasconducted among 160 women of the reproductive age group ranging from 26 to 45 years of age at Aashakiran fertility world which is situated in the state of Punjab's GILLCO VALLEY, KHARAR. over a period of one and half years. Four groups bifurcated as 26-30, 31-35,36-40, 41-45 age included 40 patients each thus makinga total of 160. The study took over a period of one and half years for completion. The diagnosis was made on the basis of physical, biochemical and Nugent’s Scoring method. Out of the total 160 women enrolled in the study 90 women showed positive signs of infection and majority of the infected females belonged to 26-30 age group.