H. pylori infection is the most frequent around the globe, affecting 40–50% of the population in industrialized nations and 80–90% of the population in underdeveloped nations. The former categorization method recognized H. pylori PCR by culture, histology, or non-invasive tests such as stool sample antigen, UBT, or serology. While helpful, each of these tests has limits and may provide erroneous findings. Studies show that the Rapid Urease Test (RUT) is a simple, non-invasive method for H. pylori infection identification because of its high sensitivity and specificity. This study examined the Rapid Urease Test's sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) in detecting H. pylori infection following a stomach biopsy. Finally, the RUT reliably detects H. pylori infection and can replace histopathology in treating acid peptic illness and dyspepsia