Infection is a significant concern in healthcare facilities globally. Healthcare personnel, such as medical laboratory technicians and nurses, are susceptible to infection due to their proximity to diseased patients. This analysis was conducted to assess the involvement of physicians, nurses, and laboratory teams in the implementation of Infection Prevention Control (IPC) procedures among healthcare professionals (HCPs). The perspective changed from considering most Healthcare-Associated Infections (HAIs) as an inevitable expense to recognizing that the majority of them may be prevented. Hospital programs moved focus from infection management to infection prevention, necessitating prompt identification of infections, timely analysis, and active involvement in implementing treatments to reduce infections. Strategies to maintain the strong awareness, favorable attitudes, and effective Infection Prevention and Control practices by addressing the factors linked to these variables as discovered in this study should be developed and put into action.