Obesity has been defined as a global epidemic with a prevalence reaching 600million people worldwide. Obesity is also a risk factor for metabolic syndrome. Increased adipose tissue in obese individuals can cause systemic dysregulation resulting in inflammation and increased reactive oxygen species (ROS). This can cause oxidative stress. One of the markers of oxidative damage to DNA is 8-Hydroxy-Deoxyguanosine (8-OHdG). This study aims to analyze 8-OHdG levels in obese subjects and their relationship with Metabolic Syndrome. This research is analytical research using a cross-sectional research design involving 60 subjects. Each participant was examined for fasting blood sugar, HDL, and TG using blood samples and examination of 8-OHdG levels using urine samples with the ELISA method. The research results show that urine 8-OHdG levels in obese subjects had a higher value than non-obese subjects (p=0.308), urine 8-OHdG levels in obese subjects had relatively higher levels than non-metabolic syndrome subjects, although it did not have a significant value statistically (p=0.667), obesity status has a significant relationship with the incidence of metabolic syndrome where in obese people, there is a 25 times higher risk of developing metabolic syndrome than subjects without obesity (p<0.001). Based on these results, it can be concluded that there is no significant difference in urine 8-OHdG levels in obese compared to non-obese. The same is true for 8-OHdG levels in urine in obese subjects with and without metabolic syndrome.