Illegal gold mining activities still occur frequently in Buru Regency, which is one of the rivers in Buru Regency, Maluku. This activity causes environmental pollution in the form of mercury (Hg) and cyanide (CN). The purpose of this study was to determine Hg and CN pollution in the Anahoni River, Buru Regency. The results showed that Hg metal was detected in water with concentrations between 0.0003-0.0069 mg/L and sediment concentrations between 0.055-0.209 mg/kg. Meanwhile, CN metal was detected in water other than stations 9 and 10 of 0.002 mg/L and sediment detected at all stations ranged from 0.012-0.326 mg/kg. Statistical analysis shows that the concentration of Hg in river water at stations 3 and 4 has passed the quality standard and the sediment is not above the quality standard,