Formation of atherosclerotic plaques is the major cause of coronary artery disease (CAD). Oxidative stresses which consume smooth muscle cells and macrophages for the formation of foam cells, is a significant factor in the development of atherosclerosis. A pro-oxidant and anti-oxidant imbalance can produce highly reactive and unstable free radicals; which target proteins and DNA. Degradation of Polyunsaturated lipids by reactive oxygen species produces malondialdehyde (MDA) is a potential biochemical marker. Antioxidant enzyme activity is also decreased in CHD, which is associated with a higher risk of developing the disease. The hospital based cross sectional observational study was conducted to evaluate the Level of oxidative stress markers like malondialdehyde (MDA) & antioxidants markers superoxide dismutase (SOD), catalase (CAT) in premature coronary artery disease (CAD) patients & compared with healthy individuals.