Development of a simple, accurate, time-tested, high-performance liquid chromatography method for determination of Allopurinol in bulk and Pharmaceutical Formulation. HPLC system used was JASCO system equipped with model PU 4180 RHPLC pump, Rheodyne sample injection port (20 μl), JASCO UV-4075 UV-VIS detector. Separation was carried out on HiQSil C18 (250 mm × 4.6 mm, 5 µm) column using mixture of acetonitrile and ammonium acetate buffer (20 mM) in the ratio of 55:45, v/v as mobile phase at flow rate of 1.0 mL/min. Samples were injected using Rheodyne injector with 20 µL loop, Detection was carried out at 254nm. The retention time was found to be 3.05 min. The HPLC linear regression analysis results for calibration plots demonstrated a good relationship with (r2 = 0.9992). The method has been validated for its accuracy, Recovery, Robustness and documented. The LOD and LOQ were found to be 1.52 & 2.55 μg/m/ respectively for API and 1.78 & 2.78 μg/ml respectively for tablet. The proposed HPLC method has been proven to be effective for the determination of Allopurinol in Bulk as well as pharmaceutical dosage forms.