A simple and accurate method was developed for the determination and validation of the Econazole Nitrate. HPLC system used was JASCO system equipped with model PU 4180 RHPLC pump, Rheodyne sample injection port (20 μl), JASCO UV-4075 UV-VIS detector and ChromNAV CFR chromatography software (version 2.0). Separation was carried out on HiQSil C18 (250 mm × 4.6 mm, 5 µm) column using Acetonitrile:Methanol (85:15v/v) as mobile phase at flow rate of 1.2 mL/min. Samples were injected using Rheodyne injector with 20 µL loop, Detection was carried out at 225nm. The HPLC linear regression analysis results for calibration plots demonstrated a good relationship with (R 2 = 0.9994). The method has been validated for its accuracy, Recovery, Robustness and documented. The LOD and LOQ were found to be 1.12 & 2.59 μg/m/ respectively.