Herbal medicine has been used since ancient times for health promotion, prevention, and treatment of various disease conditions. Herbal medicines can affect the immune system in a variety of ways, such as free-radical scavenger activity, cytokine production, conversion of T cells, stimulation of antibodies, and antimicrobial actions. This study investigates the therapeutic efficacy of a herbal medicine-septilin, an anti-infective pill. The main components of Septilin tablets are amla, giloy, guggul, and liquorice. The goal is to discover and characterize possible phytochemicals from Septelin extracts using Ultraviolet–visible, Fourier transform infrared and TLC analysis. Microwave was used to extract septelin tablets by using solvent methanol: water, (70:30). Extract of septelin tablets were evaluated & confirmed the presence of phytochemicals by qualitative phytochemical analysis. The extract of the septelin tablet had 111.15± 0.055 milligram GAE/g, 55.20± 0.0064 milligram QE/g, and 6.8± 0.0015 milligram CE/g of total phenolic, flavonoid, & tannin content, respectively. UV-Vis spectrum of extract of septelin tablet represents the presence of conjugated & aromatic compounds. The existence of the group O-H, aromatic C-H starch, C=C, and C-O groups in the extract from septelin tablets was verified by the Fourier transform infrared spectrum. Thin layer chromatography with just TLC was used to develop a fingerprint for the septilin tablet