Objective: To evaluate the anticancer activity of Cassia auriculata leaf extract on Oral Squamous Cell Carcinoma cell lines. Material and methods: Biopsy of oral cancer tissues samples were taken, processed and cell lines were developed. Active component of Cassia auriculata was identified and the cell lines were treated with active component at 25µg, 50µg &100µg and Cisplatin at4µg, 6 µg &8 µg at 24,48 and 72 hours and cytotoxicity and apoptotic activity was assessed with Propidium Iodide and Acridine Orange and Ethidium Bromide staining. Results: The study revealed that the p values obtained were less than 0.05 at concentration 25µg, 50µg &100µg concentration of the test component at 24, 48 and 72 hours and on comparison the promising dose was identified to be 6 μg of Cisplatin and 50 μg/ml of the active component was sufficient to induce cytotoxicity at all 3 time periods. Significant amount of PI positive cells along with significant cells in AO/EB staining was noted. Cassia auriculata’s active component decreased the cancer cell viability with increase in concentration in a more efficient manner when compared to Cisplatin. Thus Cassia auriculata can be used as an effective anti cancer drug will lesser side effects.