The present study investigates the anti-arthritis activities of the root extract of Asteracantha longifolia. Initial assessments include an acute oral toxicity study to establish the safety profile of the extract. Subsequently, in vivo anti-arthritis activity is evaluated using two rat models: carrageenan-induced paw edema and complete Freund's adjuvant (CFA)-induced arthritis. These models are employed to assess the potential therapeutic effects of the Asteracantha longifolia root extract in inflammatory conditions. The study aims to provide valuable insights into the anti-arthritic properties of the plant extract, shedding light on its potential as a natural remedy for inflammatory joint disorders. The abstract encapsulates the primary objectives and methodologies of the research, offering a succinct overview of the study's focus and design.