For years, researchers’ attentions have focused on the use of Ti as implant materials because of their outstanding biocompatibility, high corrosion resistance and great fatigue. Ti, however, lacks mechanical strength when it is pure. Also, antibacterial activity, bioactivity and biocompatibility of Ti metals should be improved in order to utilize them as biomaterials. Niobium pentoxide (Nb2O5) coatings have shown enormous potential for usage in implants due to their superior physiochemical qualities to facilitate bone regeneration and cell proliferation. Incorporating silver (Ag) into Nb2O5 coating is an excellent way to impart the coatings antibacterial properties. In this study, we employed pulsed electrochemical deposition to co-deposit Nb2O5 and Ag simultaneously to uniformly distributed Ag particles in the coatings on Ti substrate. The Nb2O5-Ag nanocomposite coatings were chosen and subjected for in vitro study in Kokubo’s 1.5 simulated body fluid after alkaline treatment. The coatings were subjected to physico-chemical characterization, electrochemical evaluation, surface morphological and topography analyses.