Context: Although Crinum defixum Linn.(amaryllidaceae), is a bulbous herb which has a wide geographical distribution in India., possesses diverse pharmacological activities in animals, little is known about its antidepressant activity. Objective: The present study evaluated the antidepressant activities of hydroalcoholic ethanol extract of bulb of Crinum defixum (EECD) using several experimental models Materials and methods: .Adult Wistar albino rats were subjected to antidepressant activity of different animal models such as Forced swim test, Tail suspension test, Locomotor activity. Biochemical estimation of MAO, estimation of total protein Results and discussion: The effects of EECD on forced swim test performance were evaluated. The EECD (400 mg/kg, p.o.) showed dose-dependent significant results in all the methods (p < 0.001). EECD (400mg/kg) significantly (p<0.001) reduced the brain MAO-A levels compared to respected vehicle treated group. The phytochemical screening revealed the presence of alkaloid, flavonoids, β-sitosterol, and steroids. LCMS study confirms the presence of lycorine which responsible for the antidepressant activity. Conclusions: The results of the study for the first time show that the plant possesses neuropharmacological activity. Future research should focus on the identification and the antidepressant activity of the constituents from this plant.