The aim of present work is to study of antimicrobial activity of Herbal gel. The Antimicrobial herbal gel is developed with the use of Blueberry and banana Extract and tried for various microorganisms like Staphylococcus aureus, Corynebacterium, staphylococcus, Brevibacterium we found the inhibitory concentration of these microbes. Herbal medicine now become an important item for both medicinally and economically. The quality, safety, and effectiveness of these herbal products have improved along with their use. Due to the some side effects of allopathic medications, patients are becoming more and more compliant with herbal therapies. Herbal medications are considered safer than allopathic medicines as allopathic medicines are associated with the side effects. The formulas for Polyherbal Gel are created. To create an optimum formulation, the concentration of extracts in Polyherbal formulations is maintained at the chosen level while the quantity of other ingredients is adjusted. Each formulation is then evaluated to see which the best.