Azadirachta indica has been used for millennia as a traditional remedy for a multitude of human ailments. Currently, the extensive antimicrobial activities of A. indica are being explored through research in the fields of dentistry, food safety, bacteriology, mycology, virology, and parasitology. The agar diffusion test was used to check the antimicrobial activity of the A. indica extract along with Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Salmonella typhi pathogens. The values of Zone of Inhibition were tabulated according to target pathogens. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentrations (MBC) values were also recorded. A. indica extract of high antimicrobial activity against Salmonella typhi but was less active Bacillus subtilis. Moreover, the various ongoing studies and the diverse properties of A. indica discussed herein may serve as a guide for the discovery of new antimicrobials.