In recent years, the development of competent green chemistry methods for synthesis of metal nanoparticles have become a main limelight of researchers in the field of nanotechnology. Between the different biological sources, plants have been considered as the best candidate for the synthesis of metal NPs. The reasons behind choosing the plant as a best candidate among the biological sources is that plants can provide a superior platform for the synthesis of nanoparticles over chemical and biological methods, free from toxic chemicals, feasibility in cost competitive, faster rate of synthesis, protective, secondary metabolites can act as natural reducing agent as well as capping agent, and the nanoparticles from plants materials are more stable than other biological sources The current research work describes, zinc oxide nanoparticles (ZnONPs) synthesized by the leaf extracts of Laurus nobilis. The plant biomolecules induce the reduction of Zn+ ions to ZnONPs which also acts as a stabilizing agent. The prepared nanoparticles were characterized by using various analytical and spectroscopic tools such as UV visible spectroscopy, Fourier transform infrared spectroscopy (FT- IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) analysis and (EDAX). Along with this study we also investigate the antimicrobial activity of bio synthesized nanoparticles by using disc diffusion method against clinical and standard strains of the microbes Vibrio Cholerae, Staph Aureus, and Candida Albicans.