This in-vitro study aims to evaluate the antimicrobial activity of extract of natural sweetner Synsepalum dulcificum with that of xylitol against caries producing pathogen Streptococcus mutans. Synsepalum dulcificum, commonly known as miracle plant, miracle berry plant or red berry is an evergreen shrub belonging to the Sapotaceae family. It has a unique taste modifying feature that results in an increase in sweetness and decrease in sourness. This taste modifying function is due to its content, Miraculin. The taste-modifying protein, miraculin is extracted with 0.5 M NaCl solution. The various concentrations of extracts of miraculin are tested on Streptococcus mutans using agar well diffusion method. The length of inhibition zone was measured in millimeters (mm) from the edge of the well to the edge of the inhibition zone produced against the bacterial culture. The extracts were assessed in an effort to validate potential activity of the miracle fruit against the pathogen. This study reveals that the miraculin content having superior antimicrobial property at the concentration of 60 µL when compared to xylitol. This article provides an insight into the non-cariogenic property of natural sweetner miraculin. Since dental caries is a matter of concern worldwide, so sugar substitutes having non-cariogenic property can play an important role in maintaining dental health