Introduction: The goal of root canal treatment is to reduce bacteria from the infected root canal system and to avoid reinfection. The antimicrobial efficiency of MTAD (Mixture of Tetracycline Isomer, an Acid and a Detergent), Chlorhexidine, Sodium hypochlorite (NaOCl), Triphala against E. faecalis (Enterococcus faecalis) was assessed in this research. Materials and method:In this research, one hundred and fifty single-rooted extracted teeth were chosen. Samples were split randomly into five groups of thirty in each group. Teeth were immersed in 5.25percent NaOCl to eliminate surface soft tissue and debris. The teeth were divided horizontally into three sections. The middle segment was placed in a brain-heart infusion broth of E. faecalis culture. The samples were split randomly into five groups on the basis of irrigant used. Dentinal canal shavings were scrapped with a round bur on a sterile aluminum foil to test for bacterial survival. The standard deviation and mean Colony Forming units (CFU/mg) values were computed. Results:The antibacterial property of MTAD was superior to NaOCl, Chlorhexidine and Triphala againstE. faecalis. Post hoc analysis showed a significant difference in all four experimental groups Conclusion: MTAD had increased antibacterial action when compared to 2percent Chlorhexidine, 3percent Sodium hypochlorite, and Triphala against E. faecalis.