Background: The main characteristic of immune throbocytopenic purpura pathology is increased peripheral destruction of platelets, the majority of patients showing antiplatelet membrane glycoproteins antibodies. The aim of this study was to detect the role of anti-platelet glycoprotein-specific IgM and IgG antibodies in severity and prognosis of ITP. Methods: This was a prospective cohort study that was conducted at pediatric Hematology Oncology Unity, Faculty of Medicine, Zagazig University. During the study period (6 months), 5 cases/ month, 30 cases were included as a comprehensive sample. Results: In the current study, regarding to age, it ranged between 2 and 14 years with mean (7.43± 3.56). Regarding to sex, female were (56%) and male were (43.3%). In the current study, regarding to duration, it ranged between .25 and 48 years with mean (14.82± 16.49). Regarding to type of ITP, acute were (40.0%), chronic were (46.7%) and persistent were (13.3%). In this study, antiplatelet antibodies ranged between 41.19 and 244.73with mean (120.50± 59.57). Our study revealed that, regarding to treatment, IVIG was (16.7%), Nplate was (33.3%), Revolade was (26.7%), Steroid was (10.0%), Steroid&IVIG was (13.3%). In the current study, there was no statistically significant difference between type of ITP and antiplatelet antibodies. Our study showed that, there was no statistically significant difference between antiplatelet antibodies at initial and follow up. In the current study, there was statistically significant increase among PLT at initial than follow up. This study revealed that, there was statistically significant difference between petechiae & bruises and bleeding at initial and follow up. While there was no statistically significant difference between intra cranial bleeding at initial and follow up. In the current study, there was statistically significant difference between treatment at initial and follow up. Conclusion: The increased levels of antiplatelet antibodies in children with acute ITP suggest that these factors could play a role in ITP pathogenesis. Anti-platelet antibody testing could potentially be used as a rule-in test for childhood ITP, although anti-platelet antibody testing cannot be used to exclude a diagnosis of ITP.