Background: Conjunctivitis is a common health issue in India; it is now viral. It is conjunctiva inflammation that manifests as red eye. It is a contagious eye illness. Students' health education and careful management can help the disease spread. Knowledge of conjunctivitis among adolescents will aid in illness prevention. Objectives: To assess the knowledge of adolescent regarding prevention of conjunctivitis. Method: 60 adolescent were selected convenience sampling technique. Cross sectional research designs were done. Data was gathered using a structured interviewing method. Information collected for the two portions, including socio-demographic data, a knowledge questionnaire regarding prevention of conjunctivitis. Result: Showed that majority of adolescent 60% were average knowledge regarding prevention of conjunctivitis, 30% were poor knowledge regarding prevention of conjunctivitis and 10% were having good knowledge regarding prevention of conjunctivitis. Parents’ education status significant with p value less than0.05 level of significant. Conclusion: The study demonstrated that the information is necessary for adolescent regarding prevention of conjunctivitis