Background: Iron deficiency anemia (IDA) remains very significant public health worry, mainly among children aged 2-5 years. This study aims to explore the frequency of IDA in this age group, with a specific focus on the educational background of mothers, as maternal education can play a crucial role in nutritional awareness and healthcare-seeking behaviors. Aim: The main aim of our current research is to assess and associate occurrence of iron deficiency anemia in children aged 2-5 years, born to both educated and uneducated mothers attending the Pediatric Outpatient Department (OPD) at our institution. By examining the influence of maternal education, we aim to identify potential areas for targeted intervention and education programs. Methods: A cross-sectional research will be conducted connecting children aged 2-5 years attending the Pediatric OPD. Blood samples will be collected to measure hemoglobin levels, serum ferritin, and other relevant hematological parameters. A structured questionnaire will be administered to mothers, gathering information on socio-demographic factors, dietary habits, and healthcare utilization patterns. Mothers will be categorized into two groups based on their educational status (educated vs. uneducated). Results: Data analysis will involve statistical comparison of the frequency of iron deficiency anemia between the two maternal education groups. Correlation analyses will explore the association between maternal education, child nutrition, and healthcare-seeking behaviors. Subgroup analyses may be performed to identify specific risk factors within each group. The results aim to provide insights into the potential effect of maternal education on prevalence of IDA in offspring. Conclusion: This research will contribute valuable information regarding the frequency of iron deficiency anemia in children aged 2-5 years and the potential influence of maternal education. The findings may guide future interventions and policies aimed at decreasing burden of IDA in this vulnerable age group by targeting educational and healthcare resources appropriately.